Relative Quantification of Proteins and Post-Translational Modifications in Proteomic Experiments with Shared Peptides: A Weight-Based Approach

Life Sciences, Biochemistry, Bioengineering,
protein quantification   post-translational modifications   shared peptides   mass spectrometry   bioinformatics  

In proteomics research, mass spectrometry (MS) is widely used to analyze changes in protein abundance and structure. However, protein quantification faces a critical challenge: many proteins share the same peptides (shared peptides), meaning these peptides appear in the sequences of multiple proteins. Traditional methods typically rely solely on un...