In recent years, with the rapid development of gene editing tools, especially the introduction of the CRISPR-Cas9 system, it has become possible to precisely modify specific DNA sequences. Currently, the introduction of single-nucleotide variants (SNVs) as a powerful tool for studying gene function and disease associations is particularly important...

Using Genome Synteny Analysis for Strain Tracking in Complex Microbiomes Reveals Species-Specific Evolutionary Patterns Background Microbial populations differentiate into distinct strains through single-nucleotide mutations and structural variations such as recombination, insertions, and deletions. Most strain comparison methods primarily quantify...