Complete Substitution with Modified Nucleotides in Self-Amplifying RNA Suppresses the Interferon Response and Increases Potency

Life Sciences, Cell Biology, Bioengineering, Genetics,
modified nucleotides   self-amplifying RNA   interferon response   protein expression   vaccine   SARS-CoV-2  

Introduction Under the influence of the COVID-19 pandemic, mRNA vaccine technology has made significant advances. However, its short half-life and high dosage requirements have led to side effects and accessibility issues. To overcome these challenges, self-amplifying RNA (saRNA) has become a research hotspot. saRNA utilizes RNA-dependent RNA polym...