Comprehensive Pathogen Identification and Antimicrobial Resistance Prediction from Positive Blood Cultures Using Nanopore Sequencing Technology

Medicine, Infectious Diseases, Medical Laboratory Science, Life Sciences, Microbiology,
pathogen identification   antimicrobial resistance prediction   positive blood cultures   real-time sequencing   nanopore sequencing   adaptive sampling  

Report on the Research Paper: Comprehensive Pathogen Identification and Antimicrobial Resistance Prediction from Positive Blood Cultures Using Nanopore Sequencing Technology Academic Context Bloodstream infections (BSIs) are severe clinical conditions typically diagnosed through positive blood culture results and systemic signs of infection. BSIs c...

Genomic Surveillance of Multidrug-Resistant Organisms Based on Long-Read Sequencing

Medicine, Infectious Diseases, Environmental and Public Health, Life Sciences, Genetics, Microbiology,
genomic surveillance   multidrug-resistant organisms   long-read sequencing   nanopore sequencing   MLST   WGMLST   WGS  

Genomic Surveillance of Multidrug-Resistant Organisms Based on Long-Read Sequencing Research Background Multidrug-resistant organisms (MDROs) are a significant global public health threat. These organisms exhibit resistance to multiple antibiotics, making infections challenging to treat and increasing healthcare costs. To effectively monitor and co...

Grepore-seq: A Robust Workflow to Detect Changes After Gene Editing Through Long-Range PCR and Nanopore Sequencing

Life Sciences, Cell Biology, Biochemistry, Bioengineering, Genetics,
CRISPR/Cas9   genetic change   long-range PCR   nanopore sequencing   grepore-seq  

Grepore-seq: A Robust Workflow for Detecting Gene Editing Changes through Long-Range PCR and Nanopore Sequencing Research Background The CRISPR/Cas9 system, as an RNA-guided DNA endonuclease system, has been widely applied in genome editing. As its potential in clinical treatment continues to increase, comprehensive evaluation of gene editing resul...

A 39 kb Structural Variant Causing Lynch Syndrome Detected by Optical Genome Mapping and Nanopore Sequencing

Medicine, Oncology, Life Sciences, Genetics,
Lynch syndrome   optical genome mapping   nanopore sequencing   structural variant   hereditary cancer   mismatch repair genes   founder event  

Detection of a 39 kb structural variant causing Lynch syndrome using optical genome mapping and nanopore sequencing Research Background Lynch syndrome (LS) is a hereditary cancer syndrome primarily caused by pathogenic germline variants in four genes of the MMR (mismatch repair) gene family: MLH1, MSH2, MSH6, and PMS2. This syndrome is characterize...