Precise Modelling of Mitochondrial Diseases Using Optimized Mitobes

Medicine, Life Sciences, Cell Biology, Biochemistry, Genetics,
mitochondrial diseases   gene editing   Mitobes   mouse models   off-target effects   phenotypic evaluation   genetic mutations  

Precise Modeling of Mitochondrial Diseases: Research Based on Optimized Mitobes Academic Background Mitochondrial diseases are a group of genetic disorders caused by mutations in mitochondrial DNA (mtDNA), which affect cellular energy metabolism and lead to dysfunction in multiple organs. Mitochondrial DNA mutations can be homoplasmic (affecting al...

Superior Fidelity and Distinct Editing Outcomes of SaCas9 Compared with SpCas9 in Genome Editing

Medicine, Hematology, Life Sciences, Cell Biology, Biochemistry, Bioengineering, Genetics,
genome editing   sacas9   spcas9   PAM sequence   insertion efficiency   off-target effects  

Comparison of SaCas9 and SpCas9 in High-Fidelity and Different Gene Editing Outcomes Research Background CRISPR systems based on Cas9 protein have become powerful tools for genome editing, widely used in basic research and clinical gene therapy. Currently, the most commonly used Cas9 variants are SpCas9 from Streptococcus pyogenes and SaCas9 from S...

Tracking-seq Reveals the Heterogeneity of Off-target Effects in CRISPR-Cas9-mediated Genome Editing

Medicine, Basic Medical Sciences, Life Sciences, Immunology, Genetics, Biophysics, Microbiology,
CRISPR-Cas9   off-target effects   genome editing   Tracking-seq   genome editing tools  

Transcriptomics Technology Tracking-Seq Reveals Off-Target Effect Heterogeneity Mediated by CRISPR-Cas9 Research Background With the rapid development of genome editing technologies, the CRISPR-Cas9 system has been widely used in biomedical research due to its high efficiency and ease of operation. However, a significant issue with the CRISPR-Cas9 ...