Academic Background Microglia are the only resident macrophages in the central nervous system (CNS) and play critical roles in development, homeostasis, and disease. Traditionally, microglia were viewed as homogeneous “resting” or “activated” states, but the advent of single-cell sequencing technologies has revealed their remarkable transcriptional...
In the field of functional genomics, researchers have long been dedicated to predicting causal regulatory relationships from observational data. However, despite modern technologies enabling the measurement of diverse molecular modalities, inferring causal regulatory relationships from observational data remains challenging. In particular, the iden...
During tumor development, copy number alterations (CNAs) are key drivers of tumor heterogeneity and evolution. Understanding these variations is crucial for developing personalized cancer diagnostics and therapies. Single-cell sequencing technology offers the highest resolution for copy number analysis, down to the individual cell level. However, l...
Single-Cell Transcriptomics Reveals the Relationship Between Cardiac Fibroblasts and Angiogenesis: The Key Role of ANGPTL4 in HFpEF Background Introduction Heart failure is one of the major global health challenges today. Heart failure is categorized into heart failure with preserved ejection fraction (HFpEF) and heart failure with reduced ejection...
Mapping the Gene Space at Single-Cell Resolution: A Study on Gene Signal Pattern Analysis (GSPA) Academic Background Single-cell RNA sequencing (scRNA-seq) technology has made significant progress in biological research in recent years, particularly in revealing the organizational structure of the cellular state space. However, although many comput...
Multiscale Footprints Reveal the Role of Cis-Regulatory Elements in Cell Differentiation and Aging Background Introduction The regulation of gene expression is a key mechanism in cell fate determination and disease development, and cis-regulatory elements (CREs) play a crucial role in this process. CREs dynamically regulate gene expression by bindi...
Microglia and Macrophages in Glioblastoma Academic Background Glioblastoma (GBM) is the most common primary malignant tumor of the central nervous system, characterized by high invasiveness and lethality. Despite standard treatments such as surgery, chemotherapy, and radiotherapy, the survival rate of patients remains extremely limited, with a medi...
Study of Complex Mosaicism in Human Embryos: Revealing Extensive Aneuploidy During Early Embryonic Development Background and Research Objectives Pre-implantation Genetic Testing for Aneuploidy (PGT-A) is a widely used technology in in vitro fertilization (IVF) aimed at screening out abnormal embryos to improve implantation and live birth rates. Ho...
Single-Cell Atlas Reveals Immunosuppressive Microenvironment and Treg Cell Distribution in Recurrent Glioblastoma Glioblastoma is the most aggressive brain tumor, with a very high recurrence rate and poor prognosis. Although many studies have explored the tumor microenvironment of this disease, the understanding of the immune microenvironment in re...