Background Introduction Hepatocellular carcinoma (HCC) is the most common type of primary liver cancer, with a five-year survival rate of less than 20%, and treatment options are extremely limited. Traditional targeted drug therapies, such as Sorafenib and other kinase inhibitors, have been used to treat HCC, but their efficacy is limited and diffi...
Background Introduction In the field of cellular energy metabolism research, mitochondrial calcium ion ([Ca²⁺]mt) is considered an important node in regulating mitochondrial oxidative function. Its role mainly involves activating calcium-sensitive dehydrogenases in the tricarboxylic acid cycle (TCA), including isocitrate dehydrogenase (IDH) and α-k...
Optimization of Respiratory Pressure Under Mechanical Ventilation Guidance - A Study on Improving Local Lung Biomechanics Mechanical ventilation is commonly used in clinical practice to treat acute respiratory distress syndrome (ARDS) and prevent pulmonary complications after general anesthesia. However, mechanical ventilation can cause harmful str...
A Study on Self-Amplifying RNA Vaccine for Prevention of Enterovirus D68 Infection and Disease in Clinical Models 1. Background In recent years, rapid response to emerging infectious diseases and vaccine development have received much attention. Particularly, the emergence of the novel coronavirus (SARS-CoV-2) has led to rapid advancement in RNA va...
CRISPR-Cas-Based Epigenome Editing in Alzheimer’s Disease: Engineered Exosomes with a Photoinducible Protein Delivery System Background Alzheimer’s Disease (AD) is a common neurodegenerative disorder characterized by progressive decline in cognitive function and memory, with currently limited treatment options. Genome editing technologies, such as ...
Transcriptome Dynamics and Cell Dialogue Between Oocytes and Granulosa Cells During Mouse Follicle Development Overview Follicle development and maturation is a complex, multi-stage process. During this process, dynamic gene expression in oocytes and surrounding somatic cells, as well as the dialogue between them, play crucial roles. This study rev...
Benchmarking Gene Set Scoring Tools for Single-Cell ATAC-seq Data Authors: Xi Wang, Qiwei Lian, Haoyu Dong, Shuo Xu, Yaru Su, Xiaohui Wu Affiliations: Pasteurien College (Soochow University Medical College), Department of Automation, Xiamen University, School of Mathematics and Computer Science, Fuzhou University Corresponding Author: xhwu@suda.edu...
Cancer is a major global public health concern, with its complex pathological processes and diverse manifestations being a focus of research. Many studies have shown that acid-base imbalance plays a crucial role in the occurrence and development of cancer, but the underlying mechanisms are not fully understood. In this study titled “Acid–base homeo...
Grepore-seq: A Robust Workflow for Detecting Gene Editing Changes through Long-Range PCR and Nanopore Sequencing Research Background The CRISPR/Cas9 system, as an RNA-guided DNA endonuclease system, has been widely applied in genome editing. As its potential in clinical treatment continues to increase, comprehensive evaluation of gene editing resul...
Comparison of SaCas9 and SpCas9 in High-Fidelity and Different Gene Editing Outcomes Research Background CRISPR systems based on Cas9 protein have become powerful tools for genome editing, widely used in basic research and clinical gene therapy. Currently, the most commonly used Cas9 variants are SpCas9 from Streptococcus pyogenes and SaCas9 from S...