Academic Report on the Study of Layer-Specific Anatomical and Physiological Characteristics of the Retinal Neurovascular Unit Background and Problem Statement Retinal processing, similar to all neural computations, is metabolically expensive and involves the dynamic regulation of blood flow, known as neurovascular coupling (NVC). The retina is supp...
Academic Background The formation and maintenance of vascular networks are critical processes in embryonic development and tissue regeneration, involving the regulation of various biophysical forces. Endothelial cells (ECs) play a central role in angiogenesis and vasculogenesis, with the contractile forces of the cytoskeleton (actomyosin contractil...
Automated Analysis of Ultrastructure: A Study Based on Large-Scale Hyperspectral Electron Microscopy Academic Background Electron Microscopy (EM) is a key technique for studying biological ultrastructure, revealing the fine details of cells at biomolecular resolution. In recent years, advancements in automation and digitization have enabled EM to c...
Academic Background Voltage imaging is a powerful technique for studying neuronal activity, but its effectiveness is often constrained by low signal-to-noise ratios (SNR). Traditional denoising methods, such as matrix factorization, impose rigid assumptions about noise and signal structures, while existing deep learning approaches fail to fully cap...
Academic Background and Problem Statement Mitochondria, as crucial organelles in eukaryotic cells, are involved in cellular energy metabolism, signal transduction, and the regulation of cell survival and death. Mitochondrial dysfunction is associated with various human diseases, including neurodegenerative disorders, cardiovascular diseases, diabet...
Uranium-Free KMnO₄/Pb Staining Enables Imaging of Tissue Architectures in Low Vacuum Scanning Electron Microscopy Academic Background Electron Microscopy (EM) is one of the most powerful tools for studying the ultrastructure of cells and tissues. However, traditional metal staining methods for biological samples often require the use of harmful ura...
Nucleosome Fibre Topology Guides Transcription Factor Binding to Enhancers Academic Background The establishment of cellular identity relies on the concerted action of multiple transcription factors (TFs) bound to enhancers of cell-type-specific genes. Although TFs recognize specific DNA motifs within accessible chromatin, this information alone is...
Report on the Study of Diatom Phytochromes Integrating Underwater Light Spectrum to Sense Depth Academic Background The distribution of light in marine ecosystems profoundly influences aquatic life. Light not only attenuates with depth but also undergoes significant spectral changes. However, research on how phytoplankton perceive these light chang...
The Conformational Space of RNase P RNA in Solution Academic Background The conformational diversity of RNA plays a crucial role in biology, particularly in processes such as RNA splicing, packaging, cellular transcriptional activation, and responses to environmental stimuli. However, traditional biophysical techniques have been unable to directly ...
Academic Background RNA (ribonucleic acid) is a crucial molecule in living organisms, involved in various biological processes such as gene expression, regulation, and catalysis. Although a significant portion of the human genome is transcribed into RNA, the structural study of RNA molecules remains a major challenge. RNA molecules typically exhibi...