Cancer is a major global public health concern, with its complex pathological processes and diverse manifestations being a focus of research. Many studies have shown that acid-base imbalance plays a crucial role in the occurrence and development of cancer, but the underlying mechanisms are not fully understood. In this study titled “Acid–base homeo...
Grepore-seq: A Robust Workflow for Detecting Gene Editing Changes through Long-Range PCR and Nanopore Sequencing Research Background The CRISPR/Cas9 system, as an RNA-guided DNA endonuclease system, has been widely applied in genome editing. As its potential in clinical treatment continues to increase, comprehensive evaluation of gene editing resul...
Comparison of SaCas9 and SpCas9 in High-Fidelity and Different Gene Editing Outcomes Research Background CRISPR systems based on Cas9 protein have become powerful tools for genome editing, widely used in basic research and clinical gene therapy. Currently, the most commonly used Cas9 variants are SpCas9 from Streptococcus pyogenes and SaCas9 from S...
Comprehensive Characterization and Transcriptome Analysis of Terminal Erythropoiesis in Human Fetal Liver Background and Research Question Erythropoiesis is the process of red blood cell production. Initially, “primitive” erythropoiesis occurs in the yolk sac, gradually replaced by “terminal” erythropoiesis in the fetal liver (FL) and postnatal bon...
Decoding Human Biology and Disease with Single-Cell Omics Technologies Background Introduction Cells are the fundamental units of life. A single fertilized egg can develop into an entire complex human body, composed of approximately 37 trillion cells organized into various tissues, organs, and systems. Traditional cell classification methods primar...
Protein Structure Prediction: Challenges, Progress, and Shifts in Research Paradigms Protein structure prediction is an important interdisciplinary research topic that has attracted researchers from various fields including biochemistry, medicine, physics, mathematics, and computer science. Researchers have adopted multiple research paradigms to so...
Application of Homologous Recombination Deficiency (HRD) in Tumor Patient Assessment and Treatment Planning Background Homologous Recombination (HR) is an important mechanism for repairing DNA Double-Strand Breaks (DSBs). However, when key genes in the HR repair pathway are mutated or epigenetically inactivated, cells are unable to effectively repa...
Omics Perspective on Cell Fate Determination Mechanisms Background Introduction During early mammalian embryo development, a totipotent zygote undergoes several cell divisions and two rounds of cell fate determination, ultimately forming a mature blastocyst. In this process, as the embryo compresses, the establishment of apicobasal cell polarity br...
Aging-induced trnaGlu-derived fragments disrupt glutamate biosynthesis by targeting mitochondrial translation-dependent cristae organization Academic Background Mitochondrial cristae are inward protrusions of the inner mitochondrial membrane that undergo significant morphological changes during aging. However, the molecular mechanisms leading to th...
Background Introduction Metabolic dysfunction-associated fatty liver disease (MAFLD) is a globally prevalent chronic liver disease with an incidence rate of about 25%. Its prevalence is even higher among obese and type 2 diabetic populations. MAFLD is a complex systemic disease that can progress from metabolic-associated fatty liver (MAFL) to metab...