Reactivation of Insulin Receptors Can Restore Brain Function in Early Lepr-Deficient Mice Background Obesity is a chronic disease caused by excessive fat accumulation, affecting both body and brain health. Deficiency of the insulin receptor (Leptin receptor, Lepr) is considered an important factor in the pathogenesis of obesity. Insulin plays a cru...
Transcriptome Dynamics and Cell Dialogue Between Oocytes and Granulosa Cells During Mouse Follicle Development Overview Follicle development and maturation is a complex, multi-stage process. During this process, dynamic gene expression in oocytes and surrounding somatic cells, as well as the dialogue between them, play crucial roles. This study rev...
Comprehensive Single-Cell Multi-Omics Study on HIV Latency Reversal Reveals Novel Regulators of Viral Reactivation This paper, titled “Integrated single-cell multiomic analysis of HIV latency reversal reveals novel regulators of viral reactivation,” was jointly completed by Manickam Ashokkumar, Wenwen Mei, and several other researchers from institu...
Proteomic Subtyping of Small Cell Lung Cancer: Analysis of Prognosis and Treatment Strategies Research Background Small Cell Lung Cancer (SCLC) is a highly malignant and heterogeneous cancer characterized by rapid growth, early metastasis, and drug resistance, which limits treatment options and challenges prognostic prediction models. Current genom...
Benchmarking Gene Set Scoring Tools for Single-Cell ATAC-seq Data Authors: Xi Wang, Qiwei Lian, Haoyu Dong, Shuo Xu, Yaru Su, Xiaohui Wu Affiliations: Pasteurien College (Soochow University Medical College), Department of Automation, Xiamen University, School of Mathematics and Computer Science, Fuzhou University Corresponding Author: xhwu@suda.edu...
Molecular Evolution of Monkeypox Virus Protein Sequences and Codon Usage Research Background The 2022 Monkeypox virus (MPXV) outbreak has caused significant global public health concern. However, the evolutionary mechanisms of MPXV remain not fully understood. MPXV is a linear double-stranded DNA virus belonging to the family Poxviridae, subfamily ...
Cancer is a major global public health concern, with its complex pathological processes and diverse manifestations being a focus of research. Many studies have shown that acid-base imbalance plays a crucial role in the occurrence and development of cancer, but the underlying mechanisms are not fully understood. In this study titled “Acid–base homeo...
Grepore-seq: A Robust Workflow for Detecting Gene Editing Changes through Long-Range PCR and Nanopore Sequencing Research Background The CRISPR/Cas9 system, as an RNA-guided DNA endonuclease system, has been widely applied in genome editing. As its potential in clinical treatment continues to increase, comprehensive evaluation of gene editing resul...
Comparison of SaCas9 and SpCas9 in High-Fidelity and Different Gene Editing Outcomes Research Background CRISPR systems based on Cas9 protein have become powerful tools for genome editing, widely used in basic research and clinical gene therapy. Currently, the most commonly used Cas9 variants are SpCas9 from Streptococcus pyogenes and SaCas9 from S...
Comprehensive Characterization and Transcriptome Analysis of Terminal Erythropoiesis in Human Fetal Liver Background and Research Question Erythropoiesis is the process of red blood cell production. Initially, “primitive” erythropoiesis occurs in the yolk sac, gradually replaced by “terminal” erythropoiesis in the fetal liver (FL) and postnatal bon...