ZNF397 Deficiency Triggers TET2-Driven Lineage Plasticity and AR-Targeted Therapy Resistance in Prostate Cancer Academic Background and Research Motivation Existing research evidence suggests that cancer cell lineage plasticity and epigenetic reprogramming enable them to evade lineage-dependent targeted therapy by adopting lineage plasticity. Howev...

This study reveals a new targetable cancer subtype characterized by activating point mutations in the MET tyrosine kinase domain (TKD). Researchers identified MET TKD activating mutations in approximately 0.5% of patients across a genomic data set of over 600,000 cancer cases, encompassing various tumor types, with the highest prevalence in renal c...

Iron (Ferric Ion, Fe3+)-Dependent Telomerase Reactivation Drives Colorectal Cancer Development I. Research Background Colorectal Cancer (CRC) is the third most common cancer globally, involving both genetic and non-genetic factors in its pathogenesis. Existing research shows a significant association between high iron content, hereditary iron overl...

Research Background: Suicide is a global public health challenge, but there is still significant uncertainty regarding the relationship between behavioral and physiological factors and suicide attempts (SA). Previous studies often focus on limited hypothesized factors such as mental illnesses (e.g., depression), personality and psychological traits...

Bidirectional Epigenetic Editing Reveals Hierarchies in Gene Regulation Background and Research Motivation In the human genome, the role of non-coding elements such as enhancers in gene regulation is widely recognized. However, current CRISPR interference methods still have some limitations in studying non-coding elements and genetic interactions. ...

In this report, we present a detailed evaluation of a research paper published in the journal Nature Human Behaviour, titled “Principled Refinement of UK Biobank Phenotypic Data Reveals the Latent Structure of Human Variation.” The study, conducted by Caitlin E. Carey, Rebecca Shafee, Robbee Wedow, among others, was published online on XX XX, XXXX....

In recent years, with the rapid development of gene editing tools, especially the introduction of the CRISPR-Cas9 system, it has become possible to precisely modify specific DNA sequences. Currently, the introduction of single-nucleotide variants (SNVs) as a powerful tool for studying gene function and disease associations is particularly important...

Academic Background RNA isoforms play a critical role in gene expression regulation. On average, human protein-coding genes contain over eight RNA isoforms, leading to nearly four distinct protein-coding sequences. Traditional short-read sequencing technologies, due to their limitations in read length, cannot accurately assemble and quantify RNA is...

Introduction Under the influence of the COVID-19 pandemic, mRNA vaccine technology has made significant advances. However, its short half-life and high dosage requirements have led to side effects and accessibility issues. To overcome these challenges, self-amplifying RNA (saRNA) has become a research hotspot. saRNA utilizes RNA-dependent RNA polym...

In this study, we demonstrated that another type of Type II CRISPR-Cas system—the II type Cas9 system—also possesses “trans-cleavage” activity. This activity is guided by crRNA and tracrRNA and is activated via the binding of DNA or RNA targets. By combining the trans-cleavage activity of the Cas9 effector nuclease with nucleic acid amplification t...