This study reveals a new targetable cancer subtype characterized by activating point mutations in the MET tyrosine kinase domain (TKD). Researchers identified MET TKD activating mutations in approximately 0.5% of patients across a genomic data set of over 600,000 cancer cases, encompassing various tumor types, with the highest prevalence in renal c...

Iron (Ferric Ion, Fe3+)-Dependent Telomerase Reactivation Drives Colorectal Cancer Development I. Research Background Colorectal Cancer (CRC) is the third most common cancer globally, involving both genetic and non-genetic factors in its pathogenesis. Existing research shows a significant association between high iron content, hereditary iron overl...

Spatially Isolated Macrophage Populations Predict Different Outcomes in Colon Cancer Introduction Macrophages are sentinel immune cells in tissues, performing a variety of functions including pathogen defense, antigen presentation, phagocytosis of dead cells, and the secretion of signals that drive tissue repair. In tumors, macrophage infiltration ...

Academic Background In the field of plant biotechnology, traditional gene manipulation methods focus on producing the desired phenotypes and cellular activities through continuous transgene expression. However, strong continuous promoters may lead to gene silencing, metabolic burdens, or other adverse effects on yield, preventing the full realizati...

Generation of Clinically Applicable CAR-NKT Cells Research Background Chimeric Antigen Receptor (CAR) T cell therapy has been approved by the Food and Drug Administration (FDA) for the treatment of B-cell malignancies and multiple myeloma. However, the use of autologous CAR-T cell products presents challenges such as high costs, long manufacturing ...

Academic Background RNA isoforms play a critical role in gene expression regulation. On average, human protein-coding genes contain over eight RNA isoforms, leading to nearly four distinct protein-coding sequences. Traditional short-read sequencing technologies, due to their limitations in read length, cannot accurately assemble and quantify RNA is...

In this study, we demonstrated that another type of Type II CRISPR-Cas system—the II type Cas9 system—also possesses “trans-cleavage” activity. This activity is guided by crRNA and tracrRNA and is activated via the binding of DNA or RNA targets. By combining the trans-cleavage activity of the Cas9 effector nuclease with nucleic acid amplification t...

AAV-SB-CRISPR Screening Identifies Genetic Checkpoints in Tumor-Infiltrating Primary Natural Killer Cells for CAR-NK Therapy With the rapid advancement of biotechnology and gene editing technologies, increasing attention is being paid to enhancing the clinical potential of natural killer (NK) cells in cancer therapy. Despite their potential in clin...

Application of Base Editing Screening Technology in Tumor Immunotherapy T Cell Research Immune cell therapies, such as T cell transfer therapy and CAR T cell therapy, have become important clinical cancer treatment tools in certain disease fields. However, most cancer patients fail to benefit from cell therapies, possibly due to inherent changes in...

Engineering Selective Gene Drives to Steer Tumor Evolution to Counteract Drug Resistance As tumors evolve, targeted therapies against cancer often fail due to the evolution of drug resistance. This study demonstrates a way to manipulate tumor evolution repeatedly to design therapeutic opportunities, even in the presence of genetic heterogeneity. We...