Highly Efficient and Comprehensive Genome Editing Tool: A High-Performance Prime Editing Platform for Multiplexed Dropout Screening In the field of genome editing, Prime Editing has garnered significant attention for its precision and flexibility. This method allows for precise single-base substitutions and small insertions or deletions in the geno...

Exploration of Gene Set Function Discovery Using Large Language Models: GPT-4 Excels Academic Background In functional genomics, gene set enrichment analysis is a critical methodology for understanding gene functions and their associated biological processes. However, existing enrichment analyses rely heavily on curated gene function databases, suc...

ONIX: An Open-Source Platform for Multimodal Neural Recording and Perturbation During Naturalistic Behavior Research Background and Significance In recent years, significant advances have been made in large-scale neural population recording technology and studies on animal behavior in the field of neuroscience. However, these two demands often conf...

Fully Automated Microfluidic Chip-Based Embryo Sexing Technology Academic Background In the egg-laying poultry industry, newly hatched male chicks are often culled immediately after hatching, as they neither lay eggs nor produce high-quality meat. Each year, approximately 372 million male chicks are culled in the European Union. This practice has s...

Single-Molecule Optical Tweezer Technique Reveals the Binding Mechanism of a Small Molecule Activator and PR65 Protein Academic Background Protein phosphatase 2A (PP2A) is a crucial enzyme in regulating cellular signaling. Its dysfunction is closely associated with various cancers and chronic diseases, such as Alzheimer’s disease and chronic obstru...

Development of an Engineered Direct Electron Transfer Enzyme for Continuous Levodopa Monitoring Background Parkinson’s Disease (PD) is a chronic neurodegenerative disease that affects millions of people worldwide. It is characterized by the loss of dopaminergic neurons in the substantia nigra and the presence of α-synuclein aggregations forming Lew...

Advances in Paper-Based Sensors for the Detection of Antibiotic-Resistant Bacteria Background Antimicrobial resistance (AMR) is one of the most significant global public health challenges today. With the extensive use and misuse of antibiotics, more and more bacteria have developed resistance, rendering traditional treatments ineffective. According...

Early Detection of Uveal Melanoma Using Electrical Impedance Spectroscopy Academic Background Uveal Melanoma (UM) is the most common primary intraocular malignancy in adults, characterized by high aggressiveness, which can lead to vision loss and even life-threatening outcomes. Early detection and timely treatment are crucial for preserving vision ...

Resolving Native GABAA Receptor Structures from the Human Brain: A Breakthrough Study Academic Background GABAA receptors (γ-aminobutyric acid type A receptors) are among the most important inhibitory neurotransmitter receptors in the brain, responsible for regulating fast inhibitory signaling in neurons. These receptors are not only key drug targe...

Experience-Dependent Dopamine Modulation of Male Aggression in Mice Academic Background Aggression is a common social behavior in the animal kingdom, crucial for territory defense, resource competition, and mate protection. Although the role of dopamine in regulating aggression has been extensively studied, its specific neural mechanisms remain unc...