Endothelial Cells Stably Infected with Recombinant Kaposi’s Sarcoma-Associated Herpesvirus Display Distinct Viscoelastic and Morphological Properties

Kaposi’s sarcoma-associated herpesvirus (KSHV) is a γ-herpesvirus that primarily infects endothelial cells and leads to the development of Kaposi’s sarcoma (KS), especially in people living with HIV. Kaposi’s sarcoma is a malignant tumor characterized by endothelial cell proliferation, typically presenting as cutaneous lesions. After infecting endo...

High-Throughput Metabolomic Profiling of Skin Lesions: Comparative Study of Cutaneous Squamous Cell Carcinoma, Basal Cell Carcinoma, and Normal Skin via E-Biopsy Sampling

Academic Background Cutaneous Squamous Cell Carcinoma (CSCC) and Basal Cell Carcinoma (BCC) are among the most common types of skin cancer globally. Although the mortality rates of these cancers are relatively low, their incidence has been increasing year by year, significantly impacting patients’ quality of life and potentially leading to prematur...

Empowering High Throughput Screening of 3D Models: Automated Dispensing of Cervical and Endometrial Cancer Cells

Cervical cancer and endometrial cancer are significant challenges in women’s healthcare, with high mortality rates and limited treatment options making related research particularly important. Traditional two-dimensional (2D) cell screening models can provide information on the effects of drugs on single cells but fail to capture multicellular inte...

Matrix Stiffness-Mediated DNA Methylation in Endothelial Cells

In pathological conditions, alterations in tissue mechanical properties are one of the prominent features of many diseases, such as cancer. The tumor vascular system plays a critical role in tumor growth, but its structure and function often become abnormal, manifesting as disorganized, tortuous, and leaky blood vessels. Research has shown that the...

A Multicellular Mechanochemical Model to Investigate Tumor Microenvironment Remodeling and Pre-Metastatic Niche Formation

Colorectal Cancer (CRC) is one of the leading causes of cancer-related deaths in the United States, with liver metastasis being a common occurrence. Before tumor metastasis, the formation of the Pre-Metastatic Niche (PMN) is a critical process. PMN involves the activation of key liver-resident cells, including fibroblast-like stellate cells and mac...

Simulation of Somatic Evolution through the Introduction of Random Mutation to the Rules of Conway’s Game of Life

Academic Background Since its introduction in 1970, Conway’s Game of Life (GoL) has been a classic model for studying the behavior of complex systems. As a Cellular Automata (CA) model, GoL simulates the life and death of cells on a two-dimensional grid through simple rules, demonstrating complex behaviors emerging from these rules. Although GoL ha...

Artificial Intelligence in Chemical Exchange Saturation Transfer Magnetic Resonance Imaging

Academic Background Chemical Exchange Saturation Transfer (CEST) Magnetic Resonance Imaging (MRI) is an advanced non-invasive imaging technique that provides detailed molecular information about living tissues. CEST MRI works by selectively saturating exchangeable protons of specific metabolites and transferring this saturation to water molecules, ...

Targeting ADAR1 with a Small Molecule for the Treatment of Prostate Cancer

Academic Background Prostate cancer (PCA) is one of the most common malignancies in men, particularly in Western countries, where it is the second leading cause of cancer-related deaths among males. Although androgen signaling therapy is effective in the initial treatment of prostate cancer, most patients eventually relapse, and there is currently ...

Microbiome Dysbiosis, Neutrophil Recruitment and Mesenchymal Transition of Mesothelial Cells Promotes Peritoneal Metastasis of Colorectal Cancer

Background Introduction Colorectal cancer (CRC) is one of the most prevalent malignancies worldwide, with high incidence and mortality rates. According to statistics, approximately 1.87 million new cases of CRC were diagnosed globally in 2020, resulting in 915,000 deaths. Peritoneal metastasis (PM) is a common metastatic pathway of CRC, with about ...

Neutrophils Physically Interact with Tumor Cells to Form a Signaling Niche Promoting Breast Cancer Aggressiveness

Academic Background Breast cancer is one of the most common malignant tumors among women worldwide, with a complex pathogenesis involving interactions among multiple cell types and signaling pathways. In recent years, the role of the tumor microenvironment (TME) in cancer progression has gradually gained attention. Neutrophils, as an essential comp...