AAV-SB-CRISPR Screening Identifies Genetic Checkpoints in Tumor-Infiltrating Primary Natural Killer Cells for CAR-NK Therapy With the rapid advancement of biotechnology and gene editing technologies, increasing attention is being paid to enhancing the clinical potential of natural killer (NK) cells in cancer therapy. Despite their potential in clin...

Application of Base Editing Screening Technology in Tumor Immunotherapy T Cell Research Immune cell therapies, such as T cell transfer therapy and CAR T cell therapy, have become important clinical cancer treatment tools in certain disease fields. However, most cancer patients fail to benefit from cell therapies, possibly due to inherent changes in...

Engineering Selective Gene Drives to Steer Tumor Evolution to Counteract Drug Resistance As tumors evolve, targeted therapies against cancer often fail due to the evolution of drug resistance. This study demonstrates a way to manipulate tumor evolution repeatedly to design therapeutic opportunities, even in the presence of genetic heterogeneity. We...

Transcriptomics Technology Tracking-Seq Reveals Off-Target Effect Heterogeneity Mediated by CRISPR-Cas9 Research Background With the rapid development of genome editing technologies, the CRISPR-Cas9 system has been widely used in biomedical research due to its high efficiency and ease of operation. However, a significant issue with the CRISPR-Cas9 ...

Long-term Model for Studying Tumor Microenvironment Complexity: Patient-derived Mini-colons Background: The interaction between tumors and their surrounding microenvironment has profound implications for cancer development and treatment. However, existing in vitro tumor models lack the ability to capture these complex interactions. To address this ...

Single-Sample Tumor Subclonal Reconstruction Algorithm Based on Crowd-Sourced Resources Background The evolution of cancer and the genetic heterogeneity of tumors are critical fields in modern oncology research. Tumors evolve from normal cells through progressive acquisition of somatic mutations. These mutations occur probabilistically, influenced ...

Central Nervous System (CNS) autoimmune diseases, such as Neuromyelitis Optica Spectrum Disorders (NMOSD), are inflammatory demyelinating conditions characterized by relapses of optic neuritis and myelitis. Despite significant progress in the treatment of NMOSD, a subset of patients responds poorly to current therapies, with recurrent and difficult...

Oxidative Phosphorylation Regulates B Cell Effector Cytokines and Promotes Inflammation in Multiple Sclerosis Background Introduction In recent years, the antibody-independent functions of B cells in health and disease have become a research hotspot, particularly their ability to secrete different cytokines that can activate or downregulate local i...

Nucleotide Base Adducts Bind MR1 and Stimulate MR1-Restricted T Cells Abstract This article provides an overview of the latest research by Vacchini et al. on MR1-restricted T cells (MR1T cells), which discovered the presence of nucleotide base adducts as MR1T cell antigens and unveiled their metabolic pathways and physiological functions in tumor c...

TREM2 Deficiency Remodels Intestinal Macrophages and Microbiota to Enhance Anti-PD-1 Tumor Immunotherapy A research team from the Washington University School of Medicine, led by Blanda Di Luccia and other scientists, recently published a study in the journal Science Immunology, revealing how intestinal microbiota and tumor-associated macrophages i...